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Microfluidics is a technology that enables precise handling of fluids in tiny portion(typically in micro and nano scale) to make observations in  high resolution and sensitivity with  low cost and short period of time for the fluid system analysis. In human genome project, a transition from gel-based to capillary-based DNA sequencing decreased the cost of portions of human genome project by an order of magnitude and lead to many fewer errors in lane-tracking DNA sequencing. Because of this capability, the Microfluidics technology may also called lab-on-a-chip technology. Over the past ten years Microfluidics has developed from 'cool chips' used to improve the efficiency of the experiments and analysis to providing the new equipment for PCR, protein crystallization, in vitro transcription and translation and bioreactors for microbial and eukaryotic tissue culture.

 

During the Microfluidics lab, the behaviour of the fluid flow was observed through different shape of tiny channels(straight, curved, sharp edge, etc). This observation was accomplished using the up-to-date microscope(Olympus Inverted Microscope CK40 with UV Power Supply Unit U-RFLT50) with camera mounted on the top (Express-Series camera) which allows the analysis and observation of the flow on the computer monitor display. Specifically for this experiment, the flow of the fluorescent beads in ethanol solution through Microfluidic PDMS channels was examined. For the purpose of analysing the snapshots of the flow(streamlines), the Hemacytometer was used. The Hemacytometer chip was observed under different microscope focuses. This allowed for creating a size scale of the snapshots of the streamlines and overall pictures of the channels for any focus of the microscope.

Microfluidics: Observing the Fluid Flow through Different Channels

Sharp Edge ch 4X(500ms)

Sharp Edge ch 4X(500ms)

Sharpe Edge Channel with 4X magnification at Exposure Time of 500ms

Straight ch 90X

Straight ch 90X

Observing the Non-linearity in Channel Wall in 90X Magnification

Curve Corner ch (190ms).jpg

Curve Corner ch (190ms).jpg

Flow Through Curved Channel at 190ms Exposure Time

stright ch 4X

stright ch 4X

Straight Channel in 4X Magnification

Sharp Edge ch 10X(242ms)

Sharp Edge ch 10X(242ms)

Pathline of Fluorescent Beads at 242ms Exposure Time with 10X Magnification

Wide Cone ch 10X(500ms)

Wide Cone ch 10X(500ms)

Observing Flow in 10X Channel with 500ms Exposure Time

Straight ch Wall 90X(500ms)

Straight ch Wall 90X(500ms)

Clumping of the Fluid Particles on the Interior Wall of the Channel at 90X Magnification with EXposure Time of 500ms

Cone ch 10X(500ms)

Cone ch 10X(500ms)

Observing Flow rate from Wide to Narrow Channel at 10X Magnification with Exposure time of 500ms

Bubble

Bubble

Air Bubble Observed during the Examination of the Flow

90X

90X

Hemacytometer Grids at 90X Magnification

4X

4X

Hemacytometer Grids at 4X Magnification

10X

10X

Hemacytometer Grids at 10X Magnification

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